Regulatory

Part:BBa_K3032031:Design

Designed by: Denis Baronas   Group: iGEM19_Vilnius-Lithuania   (2019-10-14)


T7 based strong LexA promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LexA DNA binding sequence was carefully combined with T7 promoter sequence in order not to affect promoter's activity.



Source

This promoter sequence is design by us combining T7 promoter with LexA strong DNA binding sequence (doi:10.1093/nar/gki939).


References